Journal: Frontiers in Plant Science

Article Title: Optimizing a tomato crocin biofactory by fine-tuning plant architecture

doi: 10.3389/fpls.2025.1730399

Figure Lengend Snippet: Targeted genes and constructs. (A) Representation of the exon regions of SP and SP5G genes, and the transcriptional unit of the hygromycin resistance gene ( HygR ). The positions of the designed guide RNAs (gRNAs) are indicated with arrows. (B) Schematic representation of the T-DNA of the constructs used to target SP , SP5G , and HygR with CRISPR/Cas9. nptII , kanamycin resistance gene; SP , self-pruning.

Article Snippet: The guide RNAs (gRNAs) targeting the tomato genes were designed for Streptococcus pyogenes Cas9 using Benchling CRISPR Tool [Biology Software] (2022) with SL3.0 as the reference genome.

Techniques: Construct, CRISPR

Journal: Frontiers in Plant Science

Article Title: Optimizing a tomato crocin biofactory by fine-tuning plant architecture

doi: 10.3389/fpls.2025.1730399

Figure Lengend Snippet: CRISPR/Cas9 editing results. (A) Chromatograms of the selected sp # 4 and sp5g #6 edited lines. The gRNA sequences are underlined, and the PAM sequences are highlighted in red. The mismatches between the wild-type and mutated sequences are indicated in red in the mutated sequence. (B) Agarose gel of the PCR for HygR gene amplification. (C) Results of in vitro germination of hygr #6B seeds with and without hygromycin in the media. (D) Representation of the large deletion in hygr #6B line. TF, Tomaffron; SP , self-pruning.

Article Snippet: The guide RNAs (gRNAs) targeting the tomato genes were designed for Streptococcus pyogenes Cas9 using Benchling CRISPR Tool [Biology Software] (2022) with SL3.0 as the reference genome.

Techniques: CRISPR, Sequencing, Agarose Gel Electrophoresis, Amplification, In Vitro

Journal: Scientific Reports

Article Title: Impaired myogenesis in limb girdle muscular dystrophy type 2B

doi: 10.1038/s41598-025-10205-9

Figure Lengend Snippet: Silencing of the DYSF gene by CRISPR/Cas9 successfully reduces the synthesis of the dysferlin protein in myotubes from the control cell line. ( A ) Immunofluorescence staining indicating the absence of DYSF in the edited cell line, and the presence of myosin, showing that the cells maintain their capacity to differentiate. ( B ) Protein detection by western blotting highlights the reduction of the dysferlin protein to the levels of the LGMD2B myotubes and shows a secondary reduction of the calpain-3 protein, a finding already observed in other LGMD2B patients. Internal WB control for protein content in each sample can be observed with labelling to dystrophin (DMD), dysferlin (DYSF) and calpain3 (CAPN3).

Article Snippet: Sanger sequencing was performed to identify possible mutations and estimate the efficiency of gene editing, and we analyzed the .abi resulting files in the online software ICE CRISPR Analysis Tool (Synthego, www.synthego.com/products/bioinformatics/crispr-analysis ).

Techniques: CRISPR, Control, Immunofluorescence, Staining, Western Blot